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Characterisation of uniformly 13C, 15N labelled bacteriochlorophyll a and bacteriopheophytin a in solution and in solid state: complete assignment of the 13C, 1H and 15N chemical shifts

✍ Scribed by Tatiana Egorova-Zachernyuk; Barth van Rossum; Cees Erkelens; Huub de Groot

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
306 KB
Volume
46
Category
Article
ISSN
0749-1581

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✦ Synopsis

Abstract

In this investigation we report a complete assignment of ^13^C, ^1^H and ^15^N solution and solid state chemical shifts of two bacterial photosynthetic pigments, bacteriochlorophyll (BChl) a and bacteriopheophytin (BPheo) a. Uniform stable‐isotope labelling strategies were developed and applied to biosynthetic preparation of photosynthetic pigments, namely uniformly ^13^C, ^15^N labelled BChl a and BPheo a. Uniform stable‐isotope labelling with ^13^C, ^15^N allowed performing the assignment of the ^13^C, ^15^N and ^1^H resonances. The photosynthetic pigments were isolated from the biomass of photosynthetic bacteria Rhodopseudomonas palustris 17001 grown in uniformly ^13^C (99%) and ^15^N (98%) enriched medium. Both pigments were characterised by NMR in solution (acetone‐d~6~) and by MAS NMR in solid state and their NMR resonances were recorded and assigned through standard liquid 2D ^13^C^13^C COSY, ^1^H^13^C HMQC, ^1^H^15^N HMBC and solid 2D ^13^C^13^C RFDR, ^1^H^13^C FSLG HETCOR and ^1^H^15^N HETCOR correlation techniques at 600 MHz and 750 MHz. The characterisation of pigments is of interest from biochemical to pharmaceutical industries, photosynthesis and food research. Copyright © 2008 John Wiley & Sons, Ltd.

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