Cellular Senescence: Methods and Protocols

✍ Scribed by Marco Demaria

• Publisher: Springer New York,Humana Press
• Year: 2019
• Tongue: English
• Leaves: 256
• Series: Methods in Molecular Biology 1896
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This book describes current methods for the identification and characterization of the major hallmarks of senescent cells. Chapters focus on the high heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Additional chapters describe cellular and mouse models in which is possible to study the complex cell and non-cell autonomous functions of senescent cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cellular Senescence: Methods and Protocols aims to ensure successful results in the further study of this vital field.

✦ Table of Contents

Front Matter ....Pages i-xii
Detecting Cellular Senescence in Reprogramming (Coralie Cazin, Mathieu von Joest, Han Li)....Pages 1-10
DNA Damage In Situ Ligation Followed by Proximity Ligation Assay (DI-PLA) (Alessandro Galbiati, Fabrizio d’Adda di Fagagna)....Pages 11-20
Reactive Oxygen Species Detection in Senescent Cells (Stella Victorelli, João F. Passos)....Pages 21-29
Cellular Identification and Quantification of Senescence-Associated β-Galactosidase Activity In Vivo (Bennett G. Childs, Tyler J. Bussian, Darren J. Baker)....Pages 31-38
Relative Human Telomere Length Quantification by Real-Time PCR (A. Vasilishina, A. Kropotov, I. Spivak, A. Bernadotte)....Pages 39-44
Assessing Functional Roles of the Senescence-Associated Secretory Phenotype (SASP) (Nicolas Malaquin, Véronique Tu, Francis Rodier)....Pages 45-55
Measuring the Inflammasome in Oncogene-Induced Senescence (Irene Fernández-Duran, Núria Tarrats, Priya Hari, Juan Carlos Acosta)....Pages 57-70
Alarmin Detection in Senescent Cells (Dong Eun Kim, Albert R. Davalos)....Pages 71-81
IMR90 ER:RAS: A Cell Model of Oncogene-Induced Senescence (Andrew J. Innes, Jesús Gil)....Pages 83-92
Genotoxic Stress-Induced Senescence (Dorothy N. Y. Fan, Clemens A. Schmitt)....Pages 93-105
A Multiparametric Assay to Evaluate Senescent Cells (Hilah Gal, Ziv Porat, Valery Krizhanovsky)....Pages 107-117
A Novel Quantitative Method for the Detection of Lipofuscin, the Main By-Product of Cellular Senescence, in Fluids (Sophia V. Rizou, Konstantinos Evangelou, Vassilios Myrianthopoulos, Iordanis Mourouzis, Sophia Havaki, Aikaterini Athanasiou et al.)....Pages 119-138
Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry (Christopher D. Wiley, Sonnet Davis, Arvind Ramanathan)....Pages 139-147
Quantification of Autophagy During Senescence (Joon Tae Park, Young-Sam Lee, Sang Chul Park)....Pages 149-157
Quantification of Aneuploidy in Mammalian Systems (Hilda van den Bos, Bjorn Bakker, Aaron Taudt, Victor Guryev, Maria Colomé-Tatché, Peter M. Lansdorp et al.)....Pages 159-190
Identification of Genomic Alterations Through Multilevel DNA Structural Analysis (Ryan K. Shultzaberger, John Dresios)....Pages 191-201
Mouse Models of Accelerated Cellular Senescence (Matthew J. Yousefzadeh, Kendra I. Melos, Luise Angelini, Christin E. Burd, Paul D. Robbins, Laura J. Niedernhofer)....Pages 203-230
Methods to Quantify the NF-κB Pathway During Senescence (Lei Zhang, Jing Zhao, Aditi Gurkar, Laura J. Niedernhofer, Paul D. Robbins)....Pages 231-250
Back Matter ....Pages 251-253

✦ Subjects

Life Sciences; Cell Biology