Cellular Quiescence: Methods and Protocols

✍ Scribed by H. Daniel Lacorazza (eds.)

• Publisher: Humana Press
• Year: 2018
• Tongue: English
• Leaves: 301
• Series: Methods in Molecular Biology 1686
• Edition: 1
• Category: Library

✦ Synopsis

This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative medicine in the future. The chapters in this book were designed to address cellular quiescence in prokaryote and eukaryote organisms, detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU, H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal, neuronal, hematopoietic), genomic regulation (gene expression, transcription factors, lncRNA, RNA methylation), as well as analysis of the heterogeneity of quiescence by computer modeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cellular Quiescence: Methods and Protocols offers a broad view of basic and cutting-edge technology to inspire research in this emerging field of cell biology.

✦ Table of Contents

Front Matter ....Pages i-xi
Molecular Regulation of Cellular Quiescence: A Perspective from Adult Stem Cells and Its Niches (Wai-Kin So, Tom H. Cheung)....Pages 1-25
An In Vitro Model of Cellular Quiescence in Primary Human Dermal Fibroblasts (Mithun Mitra, Linda D. Ho, Hilary A. Coller)....Pages 27-47
Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining (Ayad Eddaoudi, Stephanie Louise Canning, Itaru Kato)....Pages 49-57
Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Cells (Hassan Azari, Loic P. Deleyrolle, Brent A. Reynolds)....Pages 59-67
Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Cycle with the FUCCI System (Alexandra Chicheportiche, Martial Ruat, François D. Boussin, Mathieu Daynac)....Pages 69-78
Determination of Histone 2B–Green Fluorescent Protein (GFP) Retention in Intestinal Stem Cells (Kevin R. Hughes, Yashwant R. Mahida)....Pages 79-89
Detecting Hematopoietic Stem Cell Proliferation Using BrdU Incorporation (Katie A. Matatall, Claudine S. Kadmon, Katherine Y. King)....Pages 91-103
Cell Cycle Analysis by Mass Cytometry (Gregory K. Behbehani)....Pages 105-124
Preparation and Analysis of Saccharomyces cerevisiae Quiescent Cells (Marla M. Spain, Sarah G. Swygert, Toshio Tsukiyama)....Pages 125-135
Identifying Quiescent Stem Cells in Hair Follicles (Christine N. Rodriguez, Hoang Nguyen)....Pages 137-147
Single EDL Myofiber Isolation for Analyses of Quiescent and Activated Muscle Stem Cells (Caroline E. Brun, Yu Xin Wang, Michael A. Rudnicki)....Pages 149-159
Investigating Cellular Quiescence of T Lymphocytes and Antigen-Induced Exit from Quiescence (Kai Yang, Hongbo Chi)....Pages 161-172
Retroviral Transduction of Quiescent Murine Hematopoietic Stem Cells (Chun Shik Park, H. Daniel Lacorazza)....Pages 173-182
Analysis of Murine Hematopoietic Stem Cell Proliferation During Inflammation (Emilie Jalbert, Eric M. Pietras)....Pages 183-200
A Facile, In Vitro 384-Well Plate System to Model Disseminated Tumor Cells in the Bone Marrow Microenvironment (Johanna M. Buschhaus, Kathryn E. Luker, Gary D. Luker)....Pages 201-213
Distinguishing States of Arrest: Genome-Wide Descriptions of Cellular Quiescence Using ChIP-Seq and RNA-Seq Analysis (Surabhi Srivastava, Hardik P. Gala, Rakesh K. Mishra, Jyotsna Dhawan)....Pages 215-239
Analysis of lncRNA-Protein Interactions by RNA-Protein Pull-Down Assays and RNA Immunoprecipitation (RIP) (Holger Bierhoff)....Pages 241-250
Analysis of MicroRNA-Mediated Translation Activation of In Vitro Transcribed Reporters in Quiescent Cells (Syed I. A. Bukhari, Samuel S. Truesdell, Shobha Vasudevan)....Pages 251-264
Genome-Wide Identification of Transcription Factor-Binding Sites in Quiescent Adult Neural Stem Cells (Shradha Mukherjee, Jenny Hsieh)....Pages 265-286
Study Quiescence Heterogeneity by Coupling Single-Cell Measurements and Computer Modeling (Jungeun Sarah Kwon, Xia Wang, Guang Yao)....Pages 287-299
Back Matter ....Pages 301-303

✦ Subjects

Cell Biology