Cellular Heterogeneity: Methods and Protocols

✍ Scribed by Natasha S. Barteneva,Ivan A. Vorobjev (eds.)

Publisher: Humana Press
Year: 2018
Tongue: English
Leaves: 363
Series: Methods in Molecular Biology 1745
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Cellular Heterogeneity: Methods and Protocols encourages readers to explore new ways of studying cells that will help lead to exciting new discoveries.

✦ Table of Contents

Front Matter ....Pages i-xvi
Front Matter ....Pages 1-1
Heterogeneity of Metazoan Cells and Beyond: To Integrative Analysis of Cellular Populations at Single-Cell Level (Natasha S. Barteneva, Ivan A. Vorobjev)....Pages 3-23
Integrating Analysis of Cellular Heterogeneity in High-Content Dose-Response Studies (Albert Gough, Tong Ying Shun, D. Lansing Taylor, Mark Schurdak)....Pages 25-46
Image-Based Tracking of Heterogeneous Single-Cell Phenotypes (Katherin Patsch, Shannon M. Mumenthaler, Daniel Ruderman)....Pages 47-63
Front Matter ....Pages 65-65
Broad Immune Monitoring and Profiling of T Cell Subsets with Mass Cytometry (Tess Melinda Brodie, Vinko Tosevski)....Pages 67-82
Spectral and Imaging Flow Cytometry in Phytoplankton Research (Veronika Dashkova, Jeff Clapper, Ivan A. Vorobjev, Natasha S. Barteneva)....Pages 83-95
X-Ray Fluorescence-Detected Flow Cytometry (Andrew M. Crawford, James E. Penner-Hahn)....Pages 97-112
Multiparametric Analysis of Myeloid Populations by Flow Cytometry (Sara A. Mathie, Alastair L. Corbin, Hayley L. Eames, Irina A. Udalova)....Pages 113-124
Quantitation of IRF3 Nuclear Translocation in Heterogeneous Cellular Populations from Cervical Tissue Using Imaging Flow Cytometry (Radiana T. Trifonova, Natasha S. Barteneva)....Pages 125-153
Methods of Study of Neuron Structural Heterogeneity: Flow Cytometry vs. Laser Interferometry (Ekaterina Kopeikina, Marina Dukhinova, Eugene D. Ponomarev)....Pages 155-166
Usage of Multiparameter Flow Cytometry to Study Microglia and Macrophage Heterogeneity in the Central Nervous System During Neuroinflammation and Neurodegeneration (Marina Dukhinova, Ekaterina Kopeikina, Eugene D. Ponomarev)....Pages 167-177
Front Matter ....Pages 179-179
Analysis of Microtubule Dynamics Heterogeneity in Cell Culture (Anara Serikbaeva, Anna Tvorogova, Sholpan Kauanova, Ivan A. Vorobjev)....Pages 181-204
Heterogeneity of Focal Adhesions and Focal Contacts in Motile Fibroblasts (Aleena Gladkikh, Anastasia Kovaleva, Anna Tvorogova, Ivan A. Vorobjev)....Pages 205-218
Laser Tweezers Raman Microspectroscopy of Single Cells and Biological Particles (Maria Navas-Moreno, James W. Chan)....Pages 219-257
Front Matter ....Pages 259-259
Quantification of the Metabolic Heterogeneity in Mycobacterial Cells Through the Measurement of the NADH/NAD+ Ratio Using a Genetically Encoded Sensor (Shabir Ahmad Bhat, Iram Khan Iqbal, Ashwani Kumar)....Pages 261-275
Characterizing Cell Heterogeneity Using PCR Fingerprinting of Surface Multigene Families in Protozoan Parasites (Víctor Seco-Hidalgo, Antonio Osuna, Luis Miguel de Pablos)....Pages 277-286
Assessing Carbon Source-Dependent Phenotypic Variability in Pseudomonas putida (Pablo I. Nikel, Víctor de Lorenzo)....Pages 287-301
Front Matter ....Pages 303-303
The Retinal Pigment Epithelial Cell Line (ARPE-19) Displays Mosaic Structural Chromosomal Aberrations (Elizaveta Fasler-Kan, Nijas Aliu, Kerstin Wunderlich, Sylvia Ketterer, Sabrina Ruggiero, Steffen Berger et al.)....Pages 305-314
FACS Isolation of Viable Cells in Different Cell Cycle Stages from Asynchronous Culture for RNA Sequencing (Daria M. Potashnikova, Sergey A. Golyshev, Alexey A. Penin, Maria D. Logacheva, Anna V. Klepikova, Anastasia A. Zharikova et al.)....Pages 315-335
Measuring Nanoscale Chromatin Heterogeneity with Partial Wave Spectroscopic Microscopy (Scott Gladstein, Andrew Stawarz, Luay M. Almassalha, Lusik Cherkezyan, John E. Chandler, Xiang Zhou et al.)....Pages 337-360
Back Matter ....Pages 361-363

✦ Subjects

Cell Biology

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