✍ Scribed by Yongfeng Guo (eds.)
No coin nor oath required. For personal study only.
This detailed volume covers a wide variety of techniques either developed specially for plant senescence studies or optimized for studying senescing plants. After an introduction to the topic, the book continues with sections on phenotypic analysis and molecular markers of plant organ senescence, hormonal control of plant senescence, stress-induced senescence, molecular and cellular processes in plant senescence, as well as systems biology approaches. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Senescence: Methods and Protocols aims to provide a useful hand book of standard protocols for plant molecular biologists working on senescence.
Front Matter ....Pages i-xii
Front Matter ....Pages 1-1
Concepts and Types of Senescence in Plants (Susheng Gan)....Pages 3-8
Initiation, Progression, and Genetic Manipulation of Leaf Senescence (Akhtar Ali, Xiaoming Gao, Yongfeng Guo)....Pages 9-31
Front Matter ....Pages 33-33
Phenotypic Analysis and Molecular Markers of Leaf Senescence (Liming Zhao, Yan Xia, Xiao-Yuan Wu, Jos H. M. Schippers, Hai-Chun Jing)....Pages 35-48
Investigation of Petal Senescence by TRV-Mediated Virus-Induced Gene Silencing in Rose (Chenxia Cheng, Junping Gao, Nan Ma)....Pages 49-63
Phenotypic Analysis and Molecular Markers of Plant Nodule Senescence (Xiuying Xia)....Pages 65-80
Quantitative Analysis of Floral Organ Abscission in Arabidopsis Via a Petal Breakstrength Assay (Chun-Lin Shi, Melinka A. Butenko)....Pages 81-88
Characterization of Climacteric and Non-Climacteric Fruit Ripening (Xiaohong Kou, Mengshi Wu)....Pages 89-102
Front Matter ....Pages 103-103
Ethylene Treatment in Studying Leaf Senescence in Arabidopsis (Zhonghai Li, Hongwei Guo)....Pages 105-112
The Assay of Abscisic Acid-Induced Stomatal Movement in Leaf Senescence (Yanyan Zhang, Kewei Zhang)....Pages 113-118
The EPR Method for Detecting Nitric Oxide in Plant Senescence (Aizhen Sun)....Pages 119-124
Hormone Treatments in Studying Leaf Senescence (Zenglin Zhang, Yongfeng Guo)....Pages 125-132
Front Matter ....Pages 133-133
Methods to Study Darkness-Induced Leaf Senescence (Yi Song, Lin Li)....Pages 135-140
Salt Treatments and Induction of Senescence (Yasuhito Sakuraba, Dami Kim, Nam-Chon Paek)....Pages 141-149
Methods for Elucidation of Plant Senescence in Response to C/N-Nutrient Balance (Shoki Aoyama, Junji Yamaguchi, Takeo Sato)....Pages 151-159
Study of Cotton Leaf Senescence Induced by Alternaria alternata Infection (Wei Liu, Wenwei Zhang, Na Zheng, Weibo Zhai, Fangjun Qi)....Pages 161-171
Study of Hydrogen Peroxide as a Senescence-Inducing Signal (Stefan Bieker, Maren Potschin, Ulrike Zentgraf)....Pages 173-193
Identification of Postharvest Senescence Regulators Through Map-Based Cloning Using Detached Arabidopsis Inflorescences as a Model Tissue (Donald A. Hunter, Rubina Jibran, Paul Dijkwel, David Chagné, Kerry Sullivan, Aakansha Kanojia et al.)....Pages 195-220
Front Matter ....Pages 221-221
Chlorophyll and Chlorophyll Catabolite Analysis by HPLC (Aditi Das, Luzia Guyer, Stefan Hörtensteiner)....Pages 223-235
Identification and Functional Analysis of Senescence-Associated Genes in Wheat (Geng Wang, Ke Li, Chunjiang Zhou)....Pages 237-246
Isolation, Purification, and Detection of Micro RNAs in Plant Senescence (Yujun Ren, Ying Miao)....Pages 247-265
In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay (Branka Uzelac, Dušica Janošević, Snežana Budimir)....Pages 267-282
Activities of Vacuolar Cysteine Proteases in Plant Senescence (Dana E. Martínez, Lorenza Costa, Juan José Guiamét)....Pages 283-297
Study of Autophagy in Plant Senescence (Xuefei Cui, Jing Zheng, Jinxin Zheng, Qingqiu Gong)....Pages 299-306
Isolation of Chloroplasts for In Organelle Protein Degradation Assay (Scott W. Vande Wetering, Judy A. Brusslan)....Pages 307-311
Plant Cell Walls: Isolation and Monosaccharide Composition Analysis (Yingzhen Kong, Malcolm O’Neill, Gongke Zhou)....Pages 313-319
Visualizing Morphological Changes of Abscission Zone Cells in Arabidopsis by Scanning Electron Microscope (Chun-Lin Shi, Melinka A. Butenko)....Pages 321-328
Front Matter ....Pages 329-329
RNA-Seq Analysis of the Transcriptome of Leaf Senescence in Tobacco (Wei Li, Yongfeng Guo)....Pages 331-337
Comprehensive Metabolomics Studies of Plant Developmental Senescence (Mutsumi Watanabe, Takayuki Tohge, Salma Balazadeh, Alexander Erban, Patrick Giavalisco, Joachim Kopka et al.)....Pages 339-358
Back Matter ....Pages 359-367
Plant Sciences
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