Cell biology of hematoporphyrin derivative (hpd)

✍ Scribed by Dr. Michael W. Berns; Marie Wilson; Robert Burns; Michael W. Berns; Alan Wile; Peter Rentzepis

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 292 KB
• Volume: 2
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/lsm.1900020309

✦ Synopsis

Tissue culture cells were treated with hematoporphyrin derivative (HPD) and analyzed for (1) H P D uptake, (2) H P D washout, and (3) fluorescence changes. The absorption peaks were the same for H P D in solution and HPD bound to cells.

HPD was taken up by all cell types rapidly within the first 10 hours ot' exposure, and leveled off by 20 hours of exposure. H P D came out of all cell types t o control levels by 72 hours after removal of the H P D bathing solution. The H P D appeared to come out of the malignant 3-T-12 cells more slowly than from the parental (nonmalignant) 3-T-3 cells. The fluorescence spectrum of HPD-treated cells changed markedly from the time that the bathing H P D solution was removed from the cells. As time progressed the weakly fluorescent 590-nm peak increased greatly and the 615-nm and 630-nm peaks decreased significantly.