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Time-resolved fluorescence microscopy of hematoporphyrin-derivative in cells

✍ Scribed by F. Docchio; R. Ramponi; C.A. Sacchi; G. Bottiroli; I. Freitas

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
444 KB
Volume
2
Category
Article
ISSN
0196-8092

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✦ Synopsis

Abstract

This work presents measurements of time‐resolved fluorescence microscopy of hematoporphyrin‐derivative (HpD) in single cells of mice tissue (both tumor and normal cells), in HeLa cells, and in solution. The measurements were performed using a pulsed‐laser microfluorometer with high spatial and temporal resolution. In agreement with the results obtained with other techniques, it has been found that the tumor cells examined present an HpD uptake about five times higher than that of the normal cells of the corresponding tissue and that, within a cell, HpD becomes localized mainly in the cytoplasm. It has also been found that the fluorescence decay time is different in cells as compared with solution, and that the presence of HpD stabilizes cell auto‐fluorescence. These results are discussed.

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