✦ LIBER ✦

Cation-induced aggregation of acidic phospholipid vesicles: the role of fatty acid unsaturation and cholesterol

✍ Scribed by Kari K. Eklund; Jouko E. Takkunen; Paavo K.J. Kinnunen

Publisher: Elsevier Science
Year: 1991
Tongue: English
Weight: 549 KB
Volume: 57
Category: Article
ISSN: 0009-3084
DOI: 10.1016/0009-3084(91)90049-h

No coin nor oath required. For personal study only.

✦ Synopsis

Cation-induced aggregation of acidic phospholipid vesicles consisting of dimyristoylphosphatidylglycerol (DMPG), dipalmitoylphosphatidylserine (DPPS), phosphatidylserine from bovine brain (brPS), and phosphatidylglycerol from egg yolk (eggPG) was studied. Significant differences were evident in the NaCl.indtured aggregation of fully saturated and unsaturated acidic phospholipid vesicles. The threshold NaCI concentration of vesicle aggregation ([NaCl]Thr) for DPPS vesicles was 320 mM compared to 610 mM observed for brPS vesicles. For DMPG vesicles the [NaCi]~r was 430 raM and no aggregation of eggPG vesicles could be observed upon addition of NaCL The threshold CaCI 2 concentrations of aggregation of DMPG and eggPG vesicles were 2.3 and 4.9 raM, respectively. The corresponding threshold CaCI 2 concentrations for DPPS and brPS vesicles were 0.85 mM and 1.3 raM, respectively. The inclusion of cholesterol into vesicles attenuated NaCI-and CaCl2-induced aggregation of DMPG and DPPS vesicles. However, enhancemeat of aggregation by inclusion of cholesterol was observed in the case of NaCl-induced aggregation of brPS vesicles. It is concluded that cation mediated membrane-membrane interactions depend, in addition to polar headgroup structure, on the fatty acid composition of the phospholipids also.

📜 SIMILAR VOLUMES

The fatty acid, phospholipid and cholest

The fatty acid, phospholipid and cholesterol content of control and polyunsaturated lamb aorta

✍ Christopher A. Pyle; Everit Payne; Douglas E. Wright; Alan H. Kirton; William M. 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 288 KB 👁 1 views

Rapid methods for the characterization o

Rapid methods for the characterization of unilamellar phospholipid vesicles. Application to studies on fatty acid donor and acceptor properties of membranes and fatty acid binding proteins

✍ M. Reers; R. Elbracht; H. Rüdel; F. Spener 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 615 KB

Vesicles having diameters from 20 to 200 nm were prepared from egg-yolk phosphatidylcholine (PC) and were separated as well as analyzed by methods that can be carried out with standard laboratory equipment. Gel-chromatography on Sephacryl S 1000 was adapted for expeditious size analysis of vesicles

Role of protein and fatty acid adsorptio

Role of protein and fatty acid adsorption on platelet adhesion and aggregation at the blood-polymer interface

✍ Kim, S. W. ;Wisniewski, S. ;Lee, E. S. ;Winn, M. L. 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 375 KB

## Abstract Thrombus formation on a foreign surface is a complicated process, involving many factors. However, there is little doubt that a foreign surface adsorbs plasma proteins upon blood contact and that the nature of this adsorbed layer may determine the mechanism of platelet adhesion and aggr

Protective role of intraperitoneally adm

Protective role of intraperitoneally administrated vitamin E and selenium on the levels of total lipid, total cholesterol, and fatty acid composition of muscle and liver tissues in rats

✍ Ökkeş Yilmaz; Sait Çelik; Mehmet Çay; Mustafa Naziroğlu 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 68 KB 👁 2 views

The aim of this work was to determine the protective effects of intraperitoneally administrated vitamin E and Se on total lipid, total cholesterol, and fatty acid composition of rat liver and muscle tissues. Total lipid content of muscle tissue in Se and combination groups decreased as compared to t

The incorporation of unsaturated fatty a

The incorporation of unsaturated fatty acids of the n-9, n-6, and n-3 families into individual phospholipids by isolated hepatocytes of thermally-acclimated rainbow trout,Salmo gairdneri

✍ Hazel, Jeffrey R. 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 English ⚖ 843 KB

Rates of incorporation of l-14C-oleic (18:ln9), -1inoleic (18:2n6), and -1inolenic (18:3n3) acids into individual phosphatides were determined in isolated hepatocytes from cold (5 "C)-and warm (20 "C)-acclimated rainbow trout, Salmo gairdneri. Fatty acid incorporation into phosphatidylcholine (PC) e

Inhibition of the mitochondrial oxidatio

Inhibition of the mitochondrial oxidation of fatty acids by tetracycline in mice and in man: Possible role in microvesicular steatosis induced by this antibiotic

✍ Eric Fréneaux; Gilles Labbe; Philippe Letteron; The Le Dinh; Claude Degott; Jean 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 831 KB

Intravenous administration of high doses of tetracycline may produce severe microvesicular steatosis of the liver in man. A similar disease is observed after ingestion of drugs which inhibit hepatic mitochondrial fatty acid @-oxidation and in subjects with various inborn defects in this metabolic pa