Rapid methods for the characterization of unilamellar phospholipid vesicles. Application to studies on fatty acid donor and acceptor properties of membranes and fatty acid binding proteins
✍ Scribed by M. Reers; R. Elbracht; H. Rüdel; F. Spener
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 615 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0009-3084
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✦ Synopsis
Vesicles having diameters from 20 to 200 nm were prepared from egg-yolk phosphatidylcholine (PC) and were separated as well as analyzed by methods that can be carried out with standard laboratory equipment. Gel-chromatography on Sephacryl S 1000 was adapted for expeditious size analysis of vesicles as well as for isolation of vesicle populations having a narrow range of diameters. The internal volume of vesicles was derived from enzymic tests for PC and for glucose encapsulated. Size analysis and enzymic determinations provided a convenient check for the lamellarity of membranes produced.
Fatty acids and fatty acid binding proteins (FABPs) must interact in vivo in tile presence of cellular membranes. As a model, interactions between unilamellar vesicles, anthroyloxypalmitic acid (A 16 : 0) and FABPs were studied with the aid of gel-chromatographic methods elabora ted and of fluorescence spectroscopy. FABP from bovine heart donated A16:0 to membranes, whereas FABP from bovine liver removed this fatty acid from vesicle membranes. The results revealed characteristic differences between cardiac and hepatic FABPs with regard to binding a fatty acid.