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Calorimetric studies of E. coli SSB protein-single-stranded DNA interactions. Effects of monovalent salts on binding enthalpy

✍ Scribed by Alexander G Kozlov; Timothy M Lohman


Book ID
115628525
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
411 KB
Volume
278
Category
Article
ISSN
0022-2836

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Interaction of E. coli Single-Stranded D
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The 3-5 single-stranded DNA (ssDNA) degrading exonuclease I of E. coli directly interacts with the E. coli ssDNA binding protein (EcoSSB). Analytical ultracentrifugation shows that all 4 carboxy-termini of an EcoSSB tetramer bind exonuclease I. Binding is weakened by increasing salt concentrations,