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Interaction of E. coli Single-Stranded DNA Binding Protein (SSB) with Exonuclease I. The Carboxy-Terminus of SSB Is the Recognition Site for the Nuclease

✍ Scribed by Genschel, Jochen; Curth, Ute; Urbanke, Claus


Book ID
118043512
Publisher
Walter de Gruyter GmbH & Co. KG
Year
2000
Tongue
English
Weight
232 KB
Volume
381
Category
Article
ISSN
1431-6730

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Interaction of E. coli Single-Stranded D
✍ Genschel, Jochen; Curth, Ute; Urbanke, Claus πŸ“‚ Article πŸ“… 2000 πŸ› Walter de Gruyter GmbH & Co. KG 🌐 English βš– 232 KB

The 3-5 single-stranded DNA (ssDNA) degrading exonuclease I of E. coli directly interacts with the E. coli ssDNA binding protein (EcoSSB). Analytical ultracentrifugation shows that all 4 carboxy-termini of an EcoSSB tetramer bind exonuclease I. Binding is weakened by increasing salt concentrations,