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Modulation of the affinity of the single-stranded DNA-binding protein of Escherichia coli (E. coli SSB) to poly(dT) by site-directed mutagenesis

✍ Scribed by Irene BAYER; Anja FLIESS; Joachim GREIPEL; Claus URBANKE; Günter MAASS


Book ID
115126142
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
595 KB
Volume
179
Category
Article
ISSN
1432-1327

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Interaction of E. coli Single-Stranded D
✍ Genschel, Jochen; Curth, Ute; Urbanke, Claus 📂 Article 📅 2000 🏛 Walter de Gruyter GmbH & Co. KG 🌐 English ⚖ 232 KB

The 3-5 single-stranded DNA (ssDNA) degrading exonuclease I of E. coli directly interacts with the E. coli ssDNA binding protein (EcoSSB). Analytical ultracentrifugation shows that all 4 carboxy-termini of an EcoSSB tetramer bind exonuclease I. Binding is weakened by increasing salt concentrations,