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Buoyant density and solvation of magnesium DNA

✍ Scribed by B. R. Vijayendran; R. D. Vold

Publisher
Wiley (John Wiley & Sons)
Year
1970
Tongue
English
Weight
674 KB
Volume
9
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

The buoyant density of T‐4 DNA was determined by equilibrium sedimentation in a density gradient, of mixed solutions of cesium and magnesium chlorides and bromides. The preferential hydration was calculated from these data, allowing appropriately for the exchange equilibrium of DNA with Cs^+^ and Mg^++^ ions. The charge and intrinsic solvation of the counterions were found to have no appreciable effect on the hydration of the DNA, the extent of solvation depending only on the thermodynamic, activity of the water. Various reasonable hypotheses are discussed to account for these results.

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