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Determination of 5′-bromodeoxyuridine in DNA by buoyant density

✍ Scribed by Daniel C. Luk; Michael D. Bick

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
244 KB
Volume
77
Category
Article
ISSN
0003-2697

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✦ Synopsis

Cesium chloride buoyant density analysis shows the density difference between the double-stranded alternating copolymers of dT and dA, poly(dAT.dAT) and of dA and BrdU, (dABU.dABU), to be 0.1730 g/cm". A mammalian DNA fully substituted with 5'-bromodeoxyuridine (BrdU) increases in density over control, unsubstituted, DNA by a value within 1% of that predicted from analysis of the copolymers. A simple relationship is presented for the accurate determination of the extent of BrdU substitution in a DNA of heterogeneous base composition.

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Inhibition of friend erythroleukemic cel
Inhibition of friend erythroleukemic cell differentiation by bromodeoxyuridine: Correlation with the amount of bromodeoxyuridine in dna
✍ Charles R. Ashman; Richard L. Davidson 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 484 KB

## Abstract These studies were undertaken to examine the relationship between the inhibition by 5‐bromodeoxyuridine (BrdU) of erythroid differentiation in Friend erythroleukemia cells and the incorporation of BrdU into DNA. Experiments were carried out in which the incorporation of BrdU into DNA an