## Abstract The pyruvate production of __E. coli__ ML 30 in continuous cultures was investigated. In a glucose mineralsalt medium with ammonium as the limiting substrate two stable stationary states (bistability) of pyruvate concentration were obtained. The bistability was limited to dilution rates
Bistabilität in der Aktivität der Glutaminsynthetase bei ammoniumlimitierten Chemostatenkulturen von Escherichia coli ML 30
✍ Scribed by Dr. P. J. Müller; I. I. Ivanova; F. Bergter
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 337 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
The approximative estimation of the function μ([NH^+^~4~]) in cultures of E. coli ML 30 had shown that bistability of the ammonium concentration in ammonium limited continuous cultures could be possible (BERGTER et al. 1977). This phenomenon suggested a bistability in the regulation of ammonia assimilation. Therefore, the activity of one key enzyme of the two ammonia assimilation systems was measured. The distribution of the activity of glutamine synthetase in ammonia limited continuous cultures after different transition states confirmed this suggestion.
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## Abstract The transient behaviour of ammonium limited continuous cultures of __E. coli__ ML 30 led to the hypothesis that the bistability of pyruvate formation primarily is caused by a bistability of the ammonia metabolism. Therefore, a function μ([NH^+^~4~]) should be expected different from tha
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