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Abhängigkeit der spezifischen Wachstumsrate von der Ammoniumkonzentration bei Escherichia coli ML 30

✍ Scribed by Prof. Dr. F. Bergter; H. Schumann; M. Koburger

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
415 KB
Volume
17
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

The transient behaviour of ammonium limited continuous cultures of E. coli ML 30 led to the hypothesis that the bistability of pyruvate formation primarily is caused by a bistability of the ammonia metabolism. Therefore, a function μ([NH^+^~4~]) should be expected different from that of MONOD type. Measurements of the specific growth rate during washout of continuous cultures at different ammonium concentrations and at such low cell concentrations that the changes in the ammonium concentration of the medium could be neglected, showed a complex function with a relative minimum near 2 mg/1 NH^+^~4~. This function allows bistability of the ammonium concentration in an ammonium limited continuous culture. The results are discussed on the basis of the two systems of ammonia assimilation found in prokaryotic cells.

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## Abstract The pyruvate production of __E. coli__ ML 30 in continuous cultures was investigated. In a glucose mineralsalt medium with ammonium as the limiting substrate two stable stationary states (bistability) of pyruvate concentration were obtained. The bistability was limited to dilution rates