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Biosynthetic uniform 13C,15N-labelling of zervamicin IIB. Complete 13C and 15N NMR assignment

✍ Scribed by Dr Tatyana V. Ovchinnikova; Zakhar O. Shenkarev; Zoya A. Yakimenko; Natalia V. Svishcheva; Andrey A. Tagaev; Dmitry A. Skladnev; Alexander S. Arseniev

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
178 KB
Volume
9
Category
Article
ISSN
1075-2617

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✦ Synopsis

Abstract

Zervamicin IIB is a member of the α‐aminoisobutyric acid containing peptaibol antibiotics. A new procedure for the biosynthetic preparation of the uniformly ^13^C‐ and ^15^N‐enriched peptaibol is described. This compound was isolated from the biomass of the fungus‐producer Emericellopsis salmosynnemata strain 336 IMI 58330 obtained upon cultivation in the totally ^13^C,^15^N‐labelled complete medium. To prepare such a medium the autolysed biomass and the exopolysaccharides of the obligate methylotrophic bacterium Methylobacillus flagellatus KT were used. This microorganism was grown in totally ^13^C,^15^N‐labelled minimal medium containing ^13^C‐methanol and ^15^N‐ammonium chloride as the only carbon and nitrogen sources. Preliminary NMR spectroscopic analysis indicated a high extent of isotope incorporation (>90%) and led to the complete ^13^C‐ and ^15^N‐NMR assignment including the stereospecific assignment of Aib residues methyl groups. The observed pattern of the structurally important secondary chemical shifts of ^1^H^α^, ^13^CO and ^13^C^α^ agrees well with the previously determined structure of zervamicin IIB in methanol solution. Copyright © 2003 European Peptide Society and John Wiley & Sons, Ltd.

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