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13C/15N distance determination by CPMAS NMR in uniformly 13C labeled molecules

✍ Scribed by Maggy Hologne; Jésus Raya; Jérôme Hirschinger

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
216 KB
Volume
44
Category
Article
ISSN
0749-1581

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✦ Synopsis

The REDOR and CPMAS techniques are applied for measuring 13C-15N dipolar coupling constants in glycine. It is shown that the selective CP or SPECIFIC CP technique removes the coherent evolution of the spin system under homonuclear 13C-13C J couplings. While the large coupling constant (approximately 900 Hz) is readily determined because of the presence of large oscillations in the CPMAS dynamics, their absence precludes the measurement of the small coupling constant (approximately 200 Hz). The experimental results and numerical simulations demonstrate that the determination of 13C-15N coupling constants of medium size (<1 kHz) by the CPMAS technique is mainly limited by the strength of the 1H decoupling field and the size of the 13C and 15N chemical shift anisotropies.

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