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Determination of Homo- and Heteronuclear Coupling Constants in Uniformly 13C,15N-Labeled DNA Oligonucleotides

✍ Scribed by D. P. Zimmer; J. P. Marino; C. Griesinger

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
805 KB
Volume
34
Category
Article
ISSN
0749-1581

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✦ Synopsis

A set of experiments to determine homo-and heteronuclear vicinal coupling constants in uniformly 13C-labeled DNA oligonucleotides is presented. 2D and 3D HCCH-E.COSY experiments were used to measure 3Jm coupling constants in the deoxyribose ring, a refocused HMBC experiment was used to measure 3Jm coupling constants about the glycosidic torsion angle x and a P-FIDS-CT-HSQC experiment was used to determine 'Jcp and 3Jm coupling constants about the backbone torsion angle E. The experiments were demonstrated on a uniformly 13C,15N-labeled 10 base pair (bp) DNA duplex which contains a dA-dT tract 4 bp in length. With these experiments it was possible to extract a number of the coupling constants from duplex DNA.

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