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Sequence-Specific Assignment of Aromatic Resonances of Uniformly 13C,15N-Labeled Proteins by Using 13C- and 15N-Edited NOESY Spectra

✍ Scribed by Zhi Lin; Yingqi Xu; Shuai Yang; Daiwen Yang

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
281 KB
Volume
118
Category
Article
ISSN
0044-8249

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We describe methods to introduce 13 C specifically to the C2 or C8 positions of 15 N-multilabeled purines, thereby permitting " 13 C tagging" of all base nitrogens except the amino group of adenosine. These procedures permit the incorporation of two or more 15 N-multilabeled monomers into a given DN