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Use of 13C tags with specifically 15N-labeled DNA and RNA

✍ Scribed by José-Luis Abad; Anthony J. Shallop; Barbara L. Gaffney; Roger A. Jones

Publisher
Wiley (John Wiley & Sons)
Year
1998
Tongue
English
Weight
196 KB
Volume
48
Category
Article
ISSN
0006-3525

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✦ Synopsis

We describe methods to introduce 13 C specifically to the C2 or C8 positions of 15 N-multilabeled purines, thereby permitting " 13 C tagging" of all base nitrogens except the amino group of adenosine. These procedures permit the incorporation of two or more 15 N-multilabeled monomers into a given DNA or RNA fragment, with and without appropriate 13 C tags. This approach increases the number of 15 N-nmr resonances that can be unambiguously distinguished and thus the amount of information from a single synthesis and a single nmr experiment.

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