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Biopolymer Synthesis on Polypropylene Supports: Oligonucleotide Arrays

✍ Scribed by R.S. Matson; J. Rampal; S.L. Pentoney; P.D. Anderson; P. Coassin

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
990 KB
Volume
224
Category
Article
ISSN
0003-2697

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✦ Synopsis

High-density oligonucleotide arrays have been constructed on clear, aminated polypropylene film using conventional phosphoramidite-based synthesis chemistries. A semiautomated 64 -channel fluidic chemical delivery system is used to prepare the (64 \times 64) array containing 4096 oligonucleotide elements. The completed array has been successfully used in the hybridization and fluorescence (Streptavidin-FITC) detection of a biotinylated 18-mer oligonucleotide target complementary to the F508 codon region of the cystic fibrosis transmembrane conductance regulator gene. A hybridization simulation model based upon Nearest Neighbor approximations is used to predict the outcome of the array hybridization experiment. 1995 Academic Press, Inc.

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