BIOLOGICAL DETERMINATION OF VITAMIN B1 (THIAMIN) IN RHIZOBIUM TRIFOLII
β Scribed by West, P. M.; Wilson, P. W.
- Book ID
- 121132954
- Publisher
- American Association for the Advancement of Science
- Year
- 1938
- Tongue
- English
- Weight
- 141 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0036-8075
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π SIMILAR VOLUMES
An in situ photochemical spectrofluorimetric flow-injection method for the determination of thiamine (vitamin B,) is proposed. It is based on the conversion of thiamine in alkaline medium into an intensively fluorescent compound. The photochemical reaction is sensitized by acetone. The determination
06 50.0 mg L β«1Χβ¬ (calibration graph: A β«Χ‘β¬ β«2310.0Χβ¬ β«Χβ¬ 0.0134 C, r β«Χ‘β¬ 0.9990, where A is the absorbance and C is the vitamin B 1 concentration in mg L β«1Χβ¬ ). Sucrose, glucose, fructose, lactose, citric acid, starch, vitamin B 2 , and vitamin B 6 do not interfere, even in concentrations five tim