Flow-injection spectrophotometric determination of vitamin B1 (thiamine) in multivitamin preparations

✍ Scribed by Clezio Aniceto; Airton Vicente Pereira; Cícero Oliveira Costa-Neto; Orlando Fatibello-Filho

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 151 KB
• Volume: 11
• Category: Article
• ISSN: 0895-7533
• DOI: 10.1002/(sici)1098-2728(1999)11:1<45::aid-lra6>3.0.co;2-7

✦ Synopsis

06 50.0 mg L ‫1מ‬ (calibration graph: A ‫ס‬ ‫2310.0מ‬ ‫ם‬ 0.0134 C, r ‫ס‬ 0.9990, where A is the absorbance and C is the vitamin B 1 concentration in mg L ‫1מ‬ ). Sucrose, glucose, fructose, lactose, citric acid, starch, vitamin B 2 , and vitamin B 6 do not interfere, even in concentrations five times higher than vitamin B 1 . Only vitamin B 12 causes interference, but this vitamin is not present in the multivitamin preparations used in this work. The detection limit was 1.0 mg L ‫1מ‬ , and the recovery of vitamin B 1 from three samples ranged from 97.5 to 105.0. The sampling rate was 41 h ‫1מ‬ and RSDs were less than 1% for solutions containing 10.0 and 30.0 mg L ‫1מ‬ vitamin B 1 (n ‫ס‬ 10). The results obtained for the determination of vitamin B 1 in commercial preparations are in good agreement with those obtained by differential pulse polarography (r ‫ס‬ 0.9999) and also with the label values (r ‫ס‬ 0.9998).