Biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism: Isolation and characterization of a mutant deficient in the activity of phosphoribosylaminoimidazole synthetase
β Scribed by Michael Irwin; Dale C. Oates; David Patterson
- Book ID
- 112558904
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 878 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1572-9931
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Ade-H and ade-I are two auxotrophic mutants of Chinese hamster ovary (CHO-K1) cells which specifically require adenine as the purine source to grow. The enzymatic defects of these mutants were examined in cell-free extracts. B was found that ade-H did not have any detectable adenylosuccinate synthet
## Abstract A mutant (A204) of Chinese hamster ovary cells (CHOβK1), which is deficient in dihydroorotate (DHO) dehydrogenase (E.C. 1.3.3.1) activity, has been isolated by a replica plating procedure. The mutant does not show a requirement for exogenously added pyrimidines. Examination of intact ce