Biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism. VI. Enzymatic studies of two mutants unable to convert inosinic acid to adenylic acid

✍ Scribed by Alice S. Tu; David Patterson

• Book ID: 104784072
• Publisher: Springer
• Year: 1977
• Tongue: English
• Weight: 861 KB
• Volume: 15
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00484561

✦ Synopsis

Ade-H and ade-I are two auxotrophic mutants of Chinese hamster ovary (CHO-K1) cells which specifically require adenine as the purine source to grow. The enzymatic defects of these mutants were examined in cell-free extracts. B was found that ade-H did not have any detectable adenylosuccinate synthetase activity and ade-I was defective in the adenylosuccinate Iyase enzyme. The relevance of adenine-requiring mutants to the study of the regulation of purine metabolism in mammalian cells" is discussed.