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Biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism: Isolation, selection, and characterization of a mutant lacking hypoxanthine-guanine phosphoribosyltransferase activity by nutritional means

✍ Scribed by David Patterson; Carol Jones


Book ID
112559321
Publisher
Springer
Year
1976
Tongue
English
Weight
730 KB
Volume
2
Category
Article
ISSN
1572-9931

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## Abstract A mutant (A204) of Chinese hamster ovary cells (CHO‐K1), which is deficient in dihydroorotate (DHO) dehydrogenase (E.C. 1.3.3.1) activity, has been isolated by a replica plating procedure. The mutant does not show a requirement for exogenously added pyrimidines. Examination of intact ce