Biochemical and physiological differentiation during morphogenesis. XXII. Observations on amino acid and protein synthesis in the cerebral cortex and liver of the newborn mouse

✍ Scribed by Flexner, Louis B. ;Flexner, Josefa B. ;Roberts, Richard B.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1958
• Tongue: English
• Weight: 899 KB
• Volume: 51
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030510306

✦ Synopsis

FIVE FIGURES

The observations reported here were made on the living, newborn mouse. An effort was made to gain estimates of the following quantities in cerebral cortex and liver: (a) the rate at which the carbon of tissue glutamic acid, a non-essential amino acid, is derived from blood glutamic acid; (b) the rate at which the carbon of several non-essential amino acids of the tissues is derived from blood glucose; (c) the rate at which the carbon of certain essential amino acids of the tissues is derived from these amino acids in the blood; and (d) the rate at which the pools of these amino acids in the tissues are drawn upon for protein synthesis. This is the first step in a study of the effect of growth and maturation on these quantities.

Methods

Glucose uniformly labeled with C14 (g1~cose-U-C~~) was prepared from BaC140,, C1400, being synthesized into sugars by Gunna leaves (Udenfriend and Gibbs, '49; Putnam and