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Bile acids. LIV—Mass spectra of conjugated bile acids

✍ Scribed by Roger Shaw; William H. Elliott


Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
522 KB
Volume
5
Category
Article
ISSN
1076-5174

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We describe an optimized liquid chromatographic method for simultaneous analysis of 10 conjugated bile acids in gall bladder and ductal bile. A quick and effective one-step purification with Sep-pak C-18 was adopted. We used a reverse phase C18 stainless steel column and an isocratic mobile phase in

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Major conjugated bile acids of human bile have been resolved by high-pressure liquid chromatography. The elutions are carried out in two stages on Corasil II or PPorasil columns; first, an alkaline solvent system (Zpropanokethyl acetate/ water/7 N ammonium hydroxide, 260:600:50:3) was used for separ

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The common conjugated bile acids of deproteinated bile from the human or the rat can be separated by high-pressure liquid chromatography and quantitated within 30 min with a 4-mm x 30-cm "fatty-acid analysis" column (Waters Associates) in 2-propanoV8.8 mM potassium phosphate buffer (pH 2.5) 160:340,