We describe an optimized liquid chromatographic method for simultaneous analysis of 10 conjugated bile acids in gall bladder and ductal bile. A quick and effective one-step purification with Sep-pak C-18 was adopted. We used a reverse phase C18 stainless steel column and an isocratic mobile phase in
Bile acids. LIV—Mass spectra of conjugated bile acids
✍ Scribed by Roger Shaw; William H. Elliott
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 522 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1076-5174
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The common conjugated bile acids of deproteinated bile from the human or the rat can be separated by high-pressure liquid chromatography and quantitated within 30 min with a 4-mm x 30-cm "fatty-acid analysis" column (Waters Associates) in 2-propanoV8.8 mM potassium phosphate buffer (pH 2.5) 160:340,