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Simultaneous determination of conjugated bile acids in human bile

✍ Scribed by Chi-Pui Pang; Shuk-Dai Mok; Ping-Kuen Lam; Jack Varma; Arthur K.C. Li

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
443 KB
Volume
23
Category
Article
ISSN
0009-9120

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✦ Synopsis

We describe an optimized liquid chromatographic method for simultaneous analysis of 10 conjugated bile acids in gall bladder and ductal bile. A quick and effective one-step purification with Sep-pak C-18 was adopted. We used a reverse phase C18 stainless steel column and an isocratic mobile phase in a flow programme and monitored the column effluent at 205 nm. The within-day CV ranged from 0.3 to 1.8%, and the between-day CV from 1.2 to 7.2%. Absolute analytical recovery ranged from 89 to 107%. Linearity ranged from 0.3 to 3 mg/mL for most bile acids. The chromatographic analysis was completed in 18 min.

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