The effect of complete sulfation of conjugated cholic, chenodeoxycholic and deoxycholic acids on bile formation was investigated in rats. The sulfated bile acids were infused intravenously in stepwise increasing doses (1, 2, 3 and 4 mumol/min/100 gm body wt) in rats after 90 min of bile acid pool de
Bile acid conjugation in the chimpanzee: effective sulfation of lithocholic acid
β Scribed by M. Schwenk; A. F. Hofmann; G. L. Carlson; J. A. Carter; F. Coulston; H. Greim
- Publisher
- Springer-Verlag
- Year
- 1978
- Tongue
- English
- Weight
- 580 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0340-5761
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were prepared by using a sulfur trioxide complex (Aldrich, Milwaukee, Wis.) as described previously (Donovon, J. M. et al., Gastroenterology 1984; 84:1046, Abstract). When sulfation was complete (all the hydroxyl groups were sulfated), the purity of sulfated bile acids was better than 96% as checked
We describe an optimized liquid chromatographic method for simultaneous analysis of 10 conjugated bile acids in gall bladder and ductal bile. A quick and effective one-step purification with Sep-pak C-18 was adopted. We used a reverse phase C18 stainless steel column and an isocratic mobile phase in