Biased sinusoidal field gel electrophoresis for the separation of large DNA
β Scribed by Dr. Tadao Kotaka; Shiro Adachi; Toshiyuki Shikata
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 860 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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## Conformational dynamics of DNA during biased sinusoidal field gel electrophoresis DNA motion during biased sinusoidal field gel electrophoresis around the antiresonance condition was investigated by direct observation using fluorescent microscopy and Brownian dynamics simulation. Time developme
It has been reported that secondary pulsed field gel (SPFG) electrophoresis can dramatically increase the speed of separation of large DNA molecules without a decrease in resolution (Zhang, T. Y., Smith, C. L., and Cantor, C. R. (1991) Nucleic Acids Res. 19, \(1291-\) 1296). However, our attempts to