Preparation of plant DNA for separation by pulsed field gel electrophoresis
β Scribed by Dr. Katrien M. Devos; Daisy Vercruysse-Dewitte
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 274 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0173-0835
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Pulsed field gradient electrophoresis allows the separation of large DNA molecules up to 2,000 kilobases (kb) in length and has the potential to close the resolution gap between standard electrophoresis of DNA molecules (smaller than 50 kb) and standard cytogenetics (larger than 2,000 kb). We have a
The velocity and orientation of T4 and X DNA have been measured for the first 20 s during pulsed-field gel electrophoresis in order to clarify the DNA motions that occur. For a square pulse with field strength E = 10 V/cm, the velocity of X DNA increases gradually to 10.5 pm/s in 1.0 s, declines to