Conformational dynamics of DNA during biased sinusoidal field gel electrophoresis

✍ Scribed by Yuichi Masubuchi; Hidehiro Oana; Mitsuhiro Matsumoto; Dr. Masao Doi; Kenichi Yoshikawa

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 1014 KB
• Volume: 17
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150170615

✦ Synopsis

Conformational dynamics of DNA during biased sinusoidal field gel electrophoresis

DNA motion during biased sinusoidal field gel electrophoresis around the antiresonance condition was investigated by direct observation using fluorescent microscopy and Brownian dynamics simulation. Time development of the center of mass velocity, v,, and the principal value of the gyration tensor, R,, was measured at this antiresonance condition. The typical stretch-contract motion, which is observed in steady field and at high frequency field, is severely suppressed, and there are two or more dominant kinks that compete with each other. Analysis of the kink motion from the simulation results supports this picture.