## Abstract The position and velocity of a band of doubleโstranded, linear DNA from bacteriophage G were measured during 120ยฐ pulsedโfield gel electrophoresis, using a video micrometer. Both the __x__ and __y__ coordinates were determined simultaneously in the plane of a 1% agarose gel; __x__ is th
Real-time velocity of DNA bands during field-inversion gel electrophoresis
โ Scribed by Chandran R. Sabanayagam; Dr. George Holzwarth
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 795 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
We study the effect of electric field intensity and agarose gel concentration on the anomalous electrophoretic mobility recently predicted. by the biased reptation model and experimentally observed for linear DNA fragments electrophoresed in continuous electric fields. We show that high fields and l
## Abstract The instantaneous position and velocity of bands of linear, doubleโstranded DNA were measured during 120ยฐ pulsedโfield electrophoresis in 1% agarose gels, using a video micrometer capable of simultaneous measurements in two dimensions. When the direction of the field was switched, the b