## Abstract The position and velocity of a band of doubleโstranded, linear DNA from bacteriophage G were measured during 120ยฐ pulsedโfield gel electrophoresis, using a video micrometer. Both the __x__ and __y__ coordinates were determined simultaneously in the plane of a 1% agarose gel; __x__ is th
Two-dimensional motion of DNA bands during pulsed-field gel electrophoresis. II. Effect of field angle
โ Scribed by Lynn M. Neitzey; George Holzwarth; Thomas Duke; Jean-Louis Viovy
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1995
- Tongue
- English
- Weight
- 826 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract The instantaneous position and velocity of bands of linear, doubleโstranded DNA were measured during 120ยฐ pulsedโfield electrophoresis in 1% agarose gels, using a video micrometer capable of simultaneous measurements in two dimensions. When the direction of the field was switched, the b
The velocity and orientation of T4 and X DNA have been measured for the first 20 s during pulsed-field gel electrophoresis in order to clarify the DNA motions that occur. For a square pulse with field strength E = 10 V/cm, the velocity of X DNA increases gradually to 10.5 pm/s in 1.0 s, declines to
A summary of the three main one-dimensional pulsed-field strategies (zero-integrated field, forward-biased field, and high frequency modulation) used for separating DNA molecules without band inversion within a preselected size range is given. Each of these strategies has size-specific features whic
## Abstract Using a computer simulation algorithm based on the reptation model, we investigated the effects of pulsed field gel electrophoresis on the separation of singleโstranded DNA molecules in denaturing polyacrylamide gels. Pulsed fields that combine two different field intensities were found