Baculovirus as versatile vectors for protein expression in insect and mammalian cells
β Scribed by Kost, Thomas A; Condreay, J Patrick; Jarvis, Donald L
- Book ID
- 109908527
- Publisher
- Nature Publishing Group
- Year
- 2005
- Tongue
- English
- Weight
- 486 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1087-0156
- DOI
- 10.1038/nbt1095
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Abbreviations AcMNPV Autographa califomica multiple nuclear polyhedrosis virus Ea Estigmene acrea ## GST glutathione S-transferase LIC ligation-independent cloning PCR polymerase chain reaction VLP virus-like particle
Baculovirus expression vectors are widely used for expressing heterologous proteins in cultured insect cells. Recent advances include further development of the system for production of multi-subunit protein complexes, co-expression of protein-modifying enzymes to improve heterologous protein produc
The cDNA encoding neuroparsin A, a polytropic neurohormone of the locust, Locusta migratoria, was inserted into the genome of Autographa californica nuclear polyhedrosis virus such that transcription was under control of the p10 promoter. A polypeptide having the same charge and the same apparent mo