Association of hepatitis C virus in human sera with β-lipoprotein

✍ Scribed by R. Thomssen; S. Bonk; C. Propfe; K.-H. Heermann; H. G. Köchel; A. Uy

• Publisher: Springer-Verlag
• Year: 1992
• Tongue: English
• Weight: 560 KB
• Volume: 181
• Category: Article
• ISSN: 0300-8584
• DOI: 10.1007/bf00198849

✦ Synopsis

Hepatitis C virus (HCV)-RNA in sera of patients with viral hepatitis C is supposed to be included, at least partially, into HCV particles. We found that the density of HCV-RNA-carrying material was variable, as determined by sucrose gradient density centrifugation (1.03-1.20g/cm3). In some of the sera examined HCV-RNA was restricted to low densities between 1.03 and 1.08 g/cm 3. In other sera additional densities of HCV-RNA were found distributed over the whole gradient with peaks at 1.12 and 1.17 and at 1.19-1.20 g/cm 3. HCV-RNA banding at low densities could be completely co-precipitated with anti-13 lipoprotein, whereas HCV-RNA fractions of higher densities were only partially precipitated or not at all. In 8 of 20 sera directly examined, HCV-RNA could be completely and in 9 sera only partially co-precipitated by anti-13 lipoprotein. In 3 sera no significant precipitation could be observed.