Assessment of glutathione-S-transferase and glutathione reductase in patients with squamous-cell carcinoma of buccal mucosa

✍ Scribed by Rakesh M. Rawal; Devendra D. Patel; Beena P. Patel; Mintoo M. Patel; Manish K. Wadhwa; Prabhudas S. Patel; Jyotsna M. Bhatavdekar

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: French
• Weight: 116 KB
• Volume: 83
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19991210)83:6<727::aid-ijc5>3.0.co;2-p

✦ Synopsis

Serum and tumor cytosolic levels of glutathione-S-transferase (GST) and glutathione-reductase (GR) activity were determined spectrophotometrically. The levels were correlated with clinicopathological criteria and a tobacco-associated protein band (T band) found in serum. The results showed significantly decreased mean serum GST levels (p F 0.02) in cancer patients as compared with controls. However, mean serum GR levels were significantly higher in patients than in controls (p F 0.01). T-band-positive patients showed low GST and low GR activity as compared with T-band-negative patients. Tumor cytosolic-enzyme levels of GST and GR activity were significantly higher (p F 0.0003 and p F 0.0001, respectively) than in corresponding adjacent noncancerous mucosa. Tumour cytosolic GST and GR activity showed significant association with clinicopathologic criteria, e.g., stage, histologic grade and nodal involvement. T-bandnegative patients showed significantly higher levels of GST (p F 0.0001) than did T-band-positive patients. Low levels of cytosolic GST may be associated with increased susceptibility towards carcinogen-induced damage. The results suggest that the presence of T band in the sera may be associated with a high-risk phenotype due to decreased detoxification ability. Int.