Aqueous polymer two-phase systems in biotechnology
✍ Scribed by Åke Gustafsson; Håkan Wennerström; Folke Tjerneld
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 318 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0378-3812
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📜 SIMILAR VOLUMES
Two-polymer aqueous phase systems are described in which ribosomes selectively partition into one of the phases. One of the phase systems is used to determine rapidly and conveniently binding of thiostrepton and erythromycin to Escherichia co/i ribosomes under equilibrium conditions.
## Abstract A proteolytic enzyme, thermolysin, was covalently immobilized to dextran which has a small number of carboxyl groups. Enzymatic synthesis of aspartame derivative (Boc‐Asp‐Phe‐OMe) using the dextran‐conjugated thermolysin was carried out in an aqueous polymer two‐phase system, where poly