Application of thiophilic membranes for the purification of monoclonal antibodies from cell culture media
✍ Scribed by U.B. Finger; J. Thömmes; D. Kinzelt; M.-R. Kula
- Book ID
- 103805217
- Publisher
- Elsevier Science
- Year
- 1995
- Weight
- 746 KB
- Volume
- 664
- Category
- Article
- ISSN
- 0378-4347
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract Monoclonal antibodies (mAbs) are used extensively for analytical, diagnostic and therapeutic applications. The purification of mAbs from cell culture supernatants typically consists of protein A, G or L affinity chromatography, often in association with other conventional chromatographi
The usefulness of thiophilic adsorption chromatography for the purification of rat IgG2b monoclonal antibodies has been evaluated. This approach has not shown specificity for immunoglobulins; therefore, to minimize potential interferences, the purification was carried out from supernatants of hybrid
Thiophilic adsorption chromatography, developed originally by Porath and colleagues (1985, FEBS Lett. 185, 306-310) for conventional chromatographic techniques, was transformed to the HPLC mode upon preparation of new and improved sulfur-containing silica beads. The new thiophilic adsorbent is of hi