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A thiophilic adsorbent for the one-step high-performance liquid chromatography purification of monoclonal antibodies

✍ Scribed by Bernhard Nopper; Fortüne Kohen; Meir Wilchek

Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
648 KB
Volume
180
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Thiophilic adsorption chromatography, developed originally by Porath and colleagues (1985, FEBS Lett. 185, 306-310) for conventional chromatographic techniques, was transformed to the HPLC mode upon preparation of new and improved sulfur-containing silica beads. The new thiophilic adsorbent is of high capacity and is suitable for the rapid and single-step purification of all subclasses of monoclonal and polyclonal antibodies. Due to its broad specificity, the thiophilic silica column is an efficient, stable, and inexpensive substitute for protein A and protein G columns used today to purify antibodies.

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