Performance of thiophilic adsorption chromatography in the purification of rat IgG2b monoclonal antibodies from serum- and protein-free culture supernatants
✍ Scribed by María Eladia Argüelles; Marta Alonso; María del Mar García Suárez; Luis Barneo; Andrés Sampedro; Juan R. de los Toyos
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 85 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0269-3879
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✦ Synopsis
The usefulness of thiophilic adsorption chromatography for the purification of rat IgG2b monoclonal antibodies has been evaluated. This approach has not shown specificity for immunoglobulins; therefore, to minimize potential interferences, the purification was carried out from supernatants of hybridomas grown in serum-and protein-free conditions. The protein purity of the six final antibody preparations assayed was always !97%. In addition to the easiness of this procedure, which enables one-step antibody purification, the materials employed are rather inexpensive and milligram amounts of antibody can be recovered from 1 L of supernatant. Overall, the purification of rat IgG2b monoclonals under the conditions reported here offers an advantageous alternative to other more expensive and cumbersome methods.