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Application of the reaction of dithioesters with ε-amino groups in lysine to the chemical modification of proteins

✍ Scribed by Jéroˆme Souppe; Martine Urrutigoity; Guy Levesque


Book ID
113267085
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
456 KB
Volume
957
Category
Article
ISSN
0167-4838

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The amount of free e-amino groups of lysine residues in proteins can be determined by a fast and straightforward method based on the use of r9F NMR spectroscopy. Unsubstituted e-amino groups are trifluoroacetylated with the reagent, S-ethyl trifluorothioacetate, in dimethyl sulfoxide solution, and t