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Anti-ovarian carcinoma anti-T3 heteroconjugates or hybrid antibodies induce tumor cell lysis by cytotoxic T-cells

✍ Scribed by Sitvana Canevari; Sylvie Ménard; Delia Mezzanzanica; Silvia Mjotti; Screnella M. Pupa; Antonio Lanzavrcchia; Maria I. Colnaghi

Publisher
John Wiley and Sons
Year
1988
Tongue
French
Weight
320 KB
Volume
41
Category
Article
ISSN
0020-7136

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✦ Synopsis

In the perspective of therapeutic in vivo targeting for T-cell attack, the monoclonal antibody (MAb) MOvI8, selected for i t s restricted reactivity with human ovarian carcinoma, and an anti-T3 MAb were used t o produce heteroconjugate or hybrid antibodies derived by fusion of relevant hybridomas. Specificity and activity of bispecific MAbs were analyzed by solid-phase RIA, immunofluorescence and a "Cr-release assay on the ovarian carcinoma cell line OVCA 432, which expresses the relevant tumor-associated antigen, and on several irrelevant tumor cell lines. Both reagents efficiently promoted, at picomolar concentration, target cell lysis by cytotoxic T-cell (CTL) clones. Although the pattern of tumor cell lines which were lysed was wider than that predicted by binding studies, further studies using a double-determinant immunoradiometric assay confirmed the specificity of MAb targeting. Analysis of reagents indicated that the hybrid MAb was superior t o the heteroaggregate as far as purification recovery and storage stability were concerned. Besides C T L clones, peripheral blood lymphocytes could also be used as cytolytic effectors, provided that a suitable in vitro activation scheme was used.

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