T cell proliferation induced by anti-cd3 antibodies: requirement for a t-t cell interaction

✍ Scribed by Jacques Bentin; John H. Vaughan; Constantine D. Tsoukas

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 690 KB
• Volume: 18
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830180421

✦ Synopsis

In the presence of interleukin 2 (IL2), soluble anti-CD3 monoclonal antibodies can stimulate highly purified normal T lymphocytes to proliferate. In these experiments HLADR' T cells constituted 13 to 20% of the total cell population, and other HLADR' cells, such as monocytes and B lymphocytes, constituted less than 1% of the population. When the HLADR' T cells were removed from the total T cell population by cytofluorometric sorting, the residual HLA DR-T cells failed to respond to soluble anti-CD3 plus IL2. When the separated HLADR' T cells were recombined with the HLADR-T cells, a response was again found. This response was dependent on the dose of HLADR' T cells added in the mixture. Irradiation individually of the HLA DR' and DR-T cells revealed that the proliferation in the cell mixture was predominantly, if not exclusively, by the HLADR-T cells. The ability of the HLADR' T cells to provide a signal necessary to this proliferation was radioresistant. These data indicate that under the conditions of these experiments HLA DR' ("activated") T cells provide a signal necessary to the responsiveness of previously resting T cells.