Oryza sativa, rice, anther culture, isolated pollen grain. Factors favouring callus proliferation and subsequent regeneration of plants from pollen grains of rice anthers (Oryza sativa L., cvs. Bahia, Girona, Balilla x Sollana and Sequial) were determined. Cultivar differences in response were foun
Anther culture of tropical japonica × indica hybrids of rice (Oryza sativaL.)
✍ Scribed by E. Guiderdoni; E. Galinato; J. Luistro; G. Vergara
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 407 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0014-2336
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✦ Synopsis
Nine japonica x indica F1 hybrids of rice involving 6 indica and 3 japonica tropical varieties, were large scale anther cultured. The frequency of callusing anthers averaged 18.7%. The microspore-derived calli produced green plants with a mean frequency of 8.7%. Albino plants represented 61% of the shoot forming calli. Monitoring of the green and albino plant regenerating capabilities of calli arising between week 4 and week 8 of incubation of the anthers showed no increase of the albino/green ratio and a slow decrease of the shoot forming ability of the transferred calli after the sixth week of culture. Spontaneous doubled haploids (SDH) represented 46% of the regenerated green plants in 4 hybrids. However, a high frequency of partially sterile regenerants was noticed among 132 SDH plants generated from a hybrid.
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