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Anther culture of tropical japonica × indica hybrids of rice (Oryza sativaL.)

✍ Scribed by E. Guiderdoni; E. Galinato; J. Luistro; G. Vergara


Publisher
Springer
Year
1992
Tongue
English
Weight
407 KB
Volume
62
Category
Article
ISSN
0014-2336

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✦ Synopsis


Nine japonica x indica F1 hybrids of rice involving 6 indica and 3 japonica tropical varieties, were large scale anther cultured. The frequency of callusing anthers averaged 18.7%. The microspore-derived calli produced green plants with a mean frequency of 8.7%. Albino plants represented 61% of the shoot forming calli. Monitoring of the green and albino plant regenerating capabilities of calli arising between week 4 and week 8 of incubation of the anthers showed no increase of the albino/green ratio and a slow decrease of the shoot forming ability of the transferred calli after the sixth week of culture. Spontaneous doubled haploids (SDH) represented 46% of the regenerated green plants in 4 hybrids. However, a high frequency of partially sterile regenerants was noticed among 132 SDH plants generated from a hybrid.


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