Totipotency of coleoptile tissue in indica rice (Oryza sativaL. cv. ch 1039)
✍ Scribed by G. S. Oinam; S. L. Kothari
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 890 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0721-7714
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✦ Synopsis
Embryogenic and non-embryogenic calluses were induced from 3,4,5 and 7d old coleoptile segments of indica rice (Oryza sativa L. cv. CH 1039). Compact, globular, yellow and creamy embryogenic and white friable non-embryogenic callus arose from the cut end and entire length of the coleoptile segments. Murashige and Skoog's (MS) medium supplemented with 2.5mg/1 2,4-D was used as callus induction medium. Plant regeneration from coleoptile segments occurred with the transfer of embryogenic callus to MS basal medium supplemented with 2.0mg/1 BAP and 0.5mg/1 NAA in combination. Average number of regenerated plants from one coleoptile ranged from9.1 to 14.0.Four day old coleoptiles showed the highest frequency of plant regeneration.