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Anther and pollen grain culture of rice (Oryza sativaL.)

✍ Scribed by M. J. Cornejo-Martin; E. Primo-Millo

Publisher: Springer
Year: 1981
Tongue: English
Weight: 404 KB
Volume: 30
Category: Article
ISSN: 0014-2336
DOI: 10.1007/bf00038780

No coin nor oath required. For personal study only.

✦ Synopsis

Oryza sativa, rice, anther culture, isolated pollen grain.

Factors favouring callus proliferation and subsequent regeneration of plants from pollen grains of rice anthers (Oryza sativa L., cvs. Bahia, Girona, Balilla x Sollana and Sequial) were determined. Cultivar differences in response were found, such as a high rate of haploid plant regeneration. '

In addition, isolated pollen grain culture was used to induce tissue proliferation outside the anther walls. The frequency ofcallus formation from isolated pollen grains was very low. It was necessary to preculture the anthers before pollen grain separation, in order to accomplish a successful development later. Root differentiation was observed in some of the obtained callus.

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