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Growth and respiration of rice (Oryza sativaL.) Cells in suspension culture

✍ Scribed by Dennis J. Brown; Harry Beevers

Publisher: Springer
Year: 1987
Tongue: English
Weight: 547 KB
Volume: 10
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00037302

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✦ Synopsis

Studies on the growth and respiration of batch suspension cultures of rice (Oryza sativa L.) in a reference medium containing Murashige-Skoog salts, 2% (w/v) sucrose and yeast extract are reported. It was found that the yeast extract contributed 70% of the phosphate in this medium, and that the cells grew equally well in continued subculture in a defined medium which contained 6 mM phosphate and 3% (w/v) sucrose and the remaining Murashige-Skoog salts. Cell clumps (up to 1.5 mm diameter) were prevalent in the initial cultures in the reference medium. In such cultures the critical 02 pressure of cell respiration was high (~ 125/~M), and ethanol accumulated. When cell clumps were routinely removed during several weekly subcultures on the defined medium cultures were obtained in which no clumps were present, the critical 02 pressures was decreased to 40/~M and no ethanol accumulated.

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